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小鼠抗雙鏈DNA抗體IgG(dsDNA)酶聯(lián)免疫吸附檢測試劑盒

ELK8414

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Mouse dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit
Reactivity:	Mouse
Alternative Names:	dsDNA Ab IgG
Assay Type:	Sandwich
Sensitivity:	0.092 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, urine and other biological fluids
Assay Length:	3.5h
Research Area:	disease
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Neuroimmune Pharmacology

STING/NF-κB/IL-6-mediated inflammation in microglia contributes to spared nerve injury (SNI)-induced neuropathic pain

日期：2021-10-20 IF：7.285
Research Square

STING/NF-κB/IL-6-mediated inflammation in microglia contributes to spared nerve injury (SNI)- induced neuropathic pain

日期：2021-02-09 IF：-
Research Square

STING/NF-κB/IL-6-mediated inflammation in microglia contributes to spared nerve injury (SNI)- induced neuropathic pain

日期：2021-02-09 IF：-
Particle and Fibre Toxicology

Short- and long-term pathologic responses to quartz are induced by nearly free silanols formed during crystal fracturing

日期：2025-01-17 IF：7.2
Particle and Fibre Toxicology

Short- and long-term pathologic responses to quartz are induced by nearly free silanols formed during crystal fracturing

日期：2025-01-17 IF：7.2
Particle and Fibre Toxicology

Short- and long-term pathologic responses to quartz are induced by nearly free silanols formed during crystal fracturing

日期：2025-01-17 IF：7.2
Particle and Fibre Toxicology

Short- and long-term pathologic responses to quartz are induced by nearly free silanols formed during crystal fracturing

日期：2025-01-17 IF：7.2

標準曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	1.948	1.855
10.00	1.608	1.515
5.00	1.027	0.934
2.50	0.828	0.735
1.25	0.572	0.479
0.63	0.356	0.263
0.32	0.228	0.135
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant dsDNA IgG and the recovery rates were calculated by comparing the measured value to the expected amount of dsDNA IgG in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	77-95%	86%
Heparin plasma(n=5)	94-106%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of dsDNA IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.