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犬尿酸(KYNA)酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK8136

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說(shuō)明 ①

Overview 文獻(xiàn)(1)

Product name:	KYNA(Kynurenic Acid) ELISA Kit
Reactivity:	General
Alternative Names:	Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine
Assay Type:	Competitive Inhibition
Sensitivity:	9.06 ng/mL
Standard:	2000 ng/mL
Detection Range:	31.25-2000 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Enzyme & Kinase;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with KYNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to KYNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KYNA in the samples is then determined by comparing the OD of the samples to the standard curve.

Biomaterials

Orally-deliverable liposome-microgel complexes dynamically remodel intestinal environment to enhance probiotic ulcerative colitis therapy via TLR4 inhibition and tryptophan metabolic crosstalk

日期：2025-05-13 IF：12.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
2000.00	0.191
1000.00	0.375
500.00	0.596
250.00	0.899
125.00	1.157
62.50	1.585
31.25	1.887
0.00	2.057

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant KYNA and the recovery rates were calculated by comparing the measured value to the expected amount of KYNA in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	87-97%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KYNA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.