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人Kruppel樣因子15(KLF15)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK4227

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(xiàn)(1)

Product name:	Human KLF15(Kruppel Like Factor 15) ELISA Kit
Reactivity:	Human
Alternative Names:	KKLF; Kidney-Enriched Kruppel-Like Factor
Assay Type:	Sandwich
Sensitivity:	0.069 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Tumor immunity;
Uniprot ID:	Q9UIH9
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

Chemical Engineering Journal

Engineered extracellular vesicles bio-nanoparticles maintain tubular cell fatty acid oxidation homeostasis to attenuate renal fibrosis

日期：2025-07-22 IF：13.2

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.201	2.119
5.00	1.642	1.560
2.50	1.175	1.093
1.25	0.912	0.830
0.63	0.457	0.375
0.32	0.323	0.241
0.16	0.226	0.144
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant KLF15 and the recovery rates were calculated by comparing the measured value to the expected amount of KLF15 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	94-107%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.