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人血小板衍生生長因子BB(PDGFBB)酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK2289

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(4)

Product name:	Human PDGFBB(Platelet Derived Growth Factor BB) ELISA Kit
Reactivity:	Human
Alternative Names:	PDGF-BB
Assay Type:	Sandwich
Sensitivity:	12.8 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFBB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFBB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFBB in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Hazardous Materials

Prenatal arsenic exposure disrupts placental angiogenesis by altering Pdgfb promoter epigenetic modification

日期：2025-06-16 IF：12.2
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets

日期：2024-03-25 IF：4.9
Cell and Tissue Banking

Lyophilization of dehydrated human amniotic membrane: a proactive approach to preserve growth factors for enhanced wound healing

日期：2025-04-17 IF：1.4
TURKISH JOURNAL OF BIOLOGY

N-cadherin mimetic hydrogels drive superior regenerative and paracrine responses in 3D cultures of adipose-derived mesenchymal stem cells

日期：2025-05-13 IF：1.1

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.963	1.878
1000.00	1.611	1.526
500.00	1.067	0.982
250.00	0.782	0.697
125.00	0.548	0.463
62.50	0.365	0.280
31.25	0.187	0.102
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PDGFBB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFBB in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	83-99%	95%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFBB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.