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人前列腺素內(nèi)過氧化物合酶2(PTGS2/COX-2)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK2096

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Reactivity:	Human
Alternative Names:	PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
Assay Type:	Sandwich
Sensitivity:	0.125 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	serum, plasma, tissue homogenates and other biological fluids
Assay length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism;
Uniprot ID:	P35354
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.126	2.022
10.00	1.628	1.524
5.00	1.136	1.032
2.50	0.864	0.760
1.25	0.589	0.485
0.63	0.319	0.215
0.32	0.249	0.145
0.00	0.104	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PTGS2/COX-2 and the recovery rates were calculated by comparing the measured value to the expected amount of PTGS2/COX-2 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-96%	87%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	78-92%	85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGS2/COX-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.