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人凝血酶原片段F1+2(F1+2)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK2028

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity:	Human
Alternative Names:	F1+2
Assay Type:	Sandwich
Sensitivity:	0.36 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Hematology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	1.964	1.869
25.00	1.616	1.521
12.50	1.052	0.957
6.25	0.733	0.638
3.13	0.548	0.453
1.57	0.315	0.220
0.79	0.230	0.135
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	96-108%	102%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.